Blar i NTNU Open på forfatter "Krog, Anne"
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Construction of XylS/Pm Expression Vectors for Bacillus subtilis - Evaluated in Bacillus subtilis and Lactobacillus plantarum
Hverven, Sara Kristine Kildal (Master thesis, 2017)Today, Escherichia coli (E.coli) is widely used as recombinant protein expression host. However, other species could be more suitable to express some recombinant proteins. In the present study, plasmids with XylS/Pm ... -
Establishing new XylS/Pm based Gram-Negative Protein Production Strains - Evaluating Pseudoalteromonas haloplanktis and Pseudomonas putida
Strypet, Goro Malene Brennmoen (Master thesis, 2018)To date, Escherichia coli is the most used bacterial host for recombinant protein production. Some proteins are, however, hard to express in this species due to a reducing intracellular environment and inclusion body ... -
Establishment and validation of the XylS/Pm regulator/promoter system in Streptomyces lividans ΔmatAB for expression and secretion of Lytic Polysaccharide Monooxygenase
Marinovic, Adrian (Master thesis, 2020)Den første generasjonen av biodrivstoff, introdusert som et miljøvennlig alternativ til fossile drivstoff, var laget av matavlinger som er en suboptimal løsning med en verdenspopulasjon som stadig utvider seg slik at ... -
Evaluation of Pseudomonas putida and Pseudoalteromonas haloplanktis as Alternative Hosts for Recombinant Gene Expression - by Evaluating the xylS/Pm Expression System with mCherry as Reporter Gene
Nygård, Thilde Sofie (Master thesis, 2017)Modern therapeutic proteins, such as insulin, are almost exclusively produced in E. coli. One of the challenges when producing recombinant proteins is solubility: a large fraction of the product is often found in an insoluble ... -
Evaluation of S. lividans ΔmatAB as a host for production of lytic polysaccharide monooxygenases
Horten, Kirsti (Master thesis, 2021)Med en stadig økende befolkning, øker også behovet for energi. I dag blir i all hovedsak dette behovet dekket med energi fra fossilt brennstoff. Selv om bruk av ikke-fornybare kilder reduseres, er det fortsatt et behov for ... -
Evaluation of the Pm/XylS expression system for production of IFN-alpha-2b in Cupriavidus necator and Escherichia coli
Bakka, Ingrid Øverlie (Master thesis, 2016)Recombinant bacteria are used for production of human proteins such as the medical important cytokine Interferon-alpha-2b (IFN-α-2b). In the present study, Escheriachia coli (E.coli) and Cupriavidus necator (C.necator) ... -
L-lysine production by Bacillus methanolicus: Genome-based mutational analysis and L-lysine secretion engineering
Nærdal, Ingemar; Netzer, Roman; Irla, Marta; Krog, Anne; Heggset, Tonje Marita Bjerkan; Wendisch, Volker F.; Brautaset, Trygve (Journal article; Peer reviewed, 2017)Bacillus methanolicus is a methylotrophic bacterium with an increasing interest in academic research and for biotechnological applications. This bacterium was previously applied for methanol-based production of l-glutamate, ... -
Metabolic engineering to understand and control productionof l-glutamate from methanol by the methylotrophic thermotolerant bacterium Bacillus methanolicus
Krog, Anne (Doktoravhandlinger ved NTNU, 1503-8181; 2013:337, Doctoral thesis, 2013)The production of L-glutamate and L-lysine together excides 3.5 million tons annually and are the most important amino acids in production volume. Their production is based on fermentation processes with the bacterium ... -
Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties
Krog, Anne; Heggeset, Tonje Marita Bjerkan; Müller, Jonas E.N.; Kupper, Christiane E.; Schneider, Olha; Vorholt, Julia A.; Ellingsen, Trond Erling; Brautaset, Trygve (Journal article; Peer reviewed, 2013-03-19)Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD+-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of ...